Techniques Workshop 2009 - Protein Sample Characterisation
This year's workshop focused on methods that can be used to test or probe the quality of protein samples, a major concern for structural biologists whether they use crystallography, NMR or cryo-electron microscopy. We had seven talks in all covering a range of methods to probe stability, folding, molecular mass and glycosylation of proteins and to examine the formation of macromolecular complexes. Techniques appropriate for both soluble and membrane proteins were discussed.
In addition to six speakers from Imperial College, we were joined by Jon Wilson from the ICR who gave a presentation on light-scattering methods. We were very glad to see that the workshop topic was as popular with the audience as the subjects of the previous two workshops - the number of attendees and questions was as high as ever!
Marko Boehm - Blue Native PAGE - A tool to analyse membrane protein complexes
Paul Hitchen - Mass Spectrometric strategies for protein characterisation
Jon Wilson - Using light scattering techniques to assess protein and protein complex quality
Rohini Rana - Rapid screening techniques to investigate membrane protein stability
Bing Liu - Protein characterisation by NMR
Patrik Kloppsteck - Optimising protein stability - a tale of buffers, light and heat
