For information on Martin Buck's research please use the link below:
http://www3.imperial.ac.uk/biology/research/plantandmicrobialsciences/projects/bucklab
My research group’s activities focus on establishing the mechanistic basis of signal perception and propagation that operates to allow the appropriate adaptive patterns of gene expression to be mounted. We study bacterial systems, of agronomic, medical and industrial relevance. We employ a range of genetic and biochemical l methodologies, often working closely with collaborators to establish the structural biology, systems biology and biophysical behaviour of the processes being investigated.
Collaborators at Imperial College:
Prof Xiaodong Zhang http://www3.imperial.ac.uk/people/xiaodong.zhang
Prof Michael Stumpf http://www3.imperial.ac.uk/theoreticalsystemsbiology/people/michaelstumpf
Dr Sivaramesh Wigneshweraraj http://www1.imperial.ac.uk/medicine/people/s.r.wig/
Dr Liming Ying http://www1.imperial.ac.uk/medicine/people/l.ying/
Prof Dick Kitney http://www3.imperial.ac.uk/people/r.kitney
Dr Edward Tate http://www3.imperial.ac.uk/people/e.tate
Collaborators outside Imperial College.
Prof Peter Stockley http://www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=PGS
Dr Roman Tuma http://www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=RT
Dr Terence Strick http://www.esf.org/activities/euryi/awards/2007/terence-strick.html
Prof Tracy Nixon http://www.bmb.psu.edu/nixon/nixon.html
Prof Yiping Wang http://www.bio.pku.edu.cn/english/faculty/wangyp/
Some recent publications (2009-2010):
Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching., Chem Commun (Camb), 2010, ISSN:1364-548X(doi) Authors:Lenn T, Gkekas CN, Bernard L, Engl C, Jovanovic G, Buck M, Ying L
Managing membrane stress: the phage shock protein (Psp) response, from molecular mechanisms to physiology., FEMS Microbiol Rev, 2010, Vol:34, Pages:797-827, ISSN:1574-6976(doi) Authors:Joly N, Engl C, Jovanovic G, Huvet M, Toni T, Sheng X, Stumpf MP, Buck M
Properties of the phage shock protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli., Microbiology, 2010, ISSN:1465-2080(doi) Authors:Jovanovic G, Engl C, Mayhew AJ, Burrows PC, Buck M
A prehydrolysis state of an AAA+ ATPase supports transcription activation of an enhancer-dependent RNA polymerase., Proc Natl Acad Sci U S A, 2010, Vol:107, Pages:9376-9381, ISSN:1091-6490(doi) Authors:Burrows PC, Joly N, Buck M.
Engineered interfaces of an AAA+ ATPase reveal a new nucleotide-dependent coordination mechanism., J Biol Chem, 2010, Vol:285, Pages:15178-15186, ISSN:1083-351X(doi) Authors:Joly N, Buck M,
A Lower-Order Oligomer Form of Phage Shock Protein A (PspA) Stably Associates with the Hexameric AAA(+) Transcription Activator Protein PspF for Negative Regulation, J MOL BIOL, 2009, Vol:394, Pages:764-775, ISSN:0022-2836(doi) Authors:Joly N, Burrows PC, Engl C, Jovanovic G, Buck M.
Physical, functional and conditional interactions between ArcAB and phage shock proteins upon secretin-induced stress in Escherichia coli., Mol Microbiol, 2009, Vol:74, Pages:16-28, ISSN:1365-2958(doi) Authors:Jovanovic G, Engl C, Buck M.
Authors:Jovanovic G, Engl C, Buck M
The role of the conserved phenylalanine in the sigma54-interacting GAFTGA motif of bacterial enhancer binding proteins., Nucleic Acids Res, 2009, Vol:37, Pages:5981-5992, ISSN:1362-4962(doi) Authors:Zhang N, Joly N, Burrows PC, Jovanovic M, Wigneshweraraj SR, Buck M.
Model-based evolutionary analysis: the natural history of phage-shock stress response., Biochem Soc Trans, 2009, Vol:37, Pages:762-767, ISSN:1470-8752(doi) Authors:Huvet M, Toni T, Tan H, Jovanovic G, Engl C, Buck M, Stumpf MP.
In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli., Mol Microbiol, 2009, Vol:73, Pages:382-396, ISSN:1365-2958(doi) Authors:Engl C, Jovanovic G, Lloyd LJ, Murray H, Spitaler M, Ying L, Errington J, Buck M.
Comparative analysis of activator-Esigma54 complexes formed with nucleotide-metal fluoride analogues., Nucleic Acids Res, 2009, Vol:37, Pages:5138-5150, ISSN:1362-4962(publication doi) Authors:Burrows PC, Joly N, Nixon BT, Buck M.
Functional roles of the pre-sensor I insertion sequence in an AAA plus bacterial enhancer binding protein, MOL MICROBIOL, 2009, Vol:73, Pages:519-533, ISSN:0950-382X(doi) Authors:Burrows PC, Schumacher J, Amartey S, Ghosh T, Burgis TA, Zhang XD, Nixon BT, Buck M.
IHF-binding sites inhibit DNA loop formation and transcription initiation., Nucleic Acids Res, 2009, Vol:37, Pages:3878-3886, ISSN:1362-4962(doi) Authors:Huo YX, Zhang YT, Xiao Y, Zhang X, Buck M, Kolb A, Wang YP.
Construction and functional analyses of a comprehensive sigma54 site-directed mutant library using alanine-cysteine mutagenesis., Nucleic Acids Res, 2009, Vol:37, Pages:4482-4497, ISSN:1362-4962(doi) Authors:Xiao Y, Wigneshweraraj SR, Weinzierl R, Wang YP, Buck M.
Coupling sigma Factor Conformation to RNA Polymerase Reorganisation for DNA Melting, J MOL BIOL, 2009, Vol:387, Pages:306-319, ISSN:0022-2836(doi) Authors:Burrows PC, Joly N, Cannon WV, Camara BP, Rappas M, Zhang XD, Dawes K, Nixon BT, Wigneshweraraj SR, Buck M.
