Flow cytometry

Flow cytometry offers the ability for a user to either analyse or isolate cells based on their phenotypic or functional properties. These properties can be defined in a number of ways – from extracellular or intracellular markers and proteins through to DNA content and cellular processes such as Calcium flux and membrane potential. Fluorescences used to detect these properties cover the spectrum from UV to far-red and are constantly increasing in versatility.

The purpose of the facility is to provide state of the art Flow Cytometry equipment and expertise. All of the analysers can be booked and used by trained personnel.

The Facility has four Flow Cytometers:

• A dual laser, 4 colour Becton Dickinson FACSCalibur analyser
• A three laser, 11 colour Becton Dickinson Fortessa analyser with HTS
• A four laser, 13 colour Becton Dickinson Fortessa analyser
• A four laser, 13 colour Becton Dickinson FACSAriaIII

We also have a computer with  FlowJo and ModFit LT software for offline analysis and presentation of data.

The facility is located on Level 3 of the Flowers Building (Calibur) and also on Level 5 of the Sir Alexander Fleming Building (Fortessas and Aria).

Please note:

All users will have to register with and be trained by Jane Srivastava before they can use the facility.