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FILM - Facility for Imaging by Light Microscopy

News

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Training courses

Currently no training courses are available in FILM. For external training courses see the LINKS page, for microscope training please get in contact with FILM staff.

FILM Club

 Our FILM Club is a meeting of facility users, for facility users, to exchange experience and techniques, get in contact, learn new tools.

  We are meeting on the last Friday of every month (except December, July and August) at 1 pm in room SAFB-120 (Fleming Building, South Kensington campus).

FILM Club calendar

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27 Jan '12 Kieran McGourty
(David Holden lab) 

Inhibition of lysosome function by Salmonella

  For this study, Kieran measured the pH inside the cytosol and lysosomes with pH-sensitive dyes. He went to great lengths to measure the pH in a reproducible and quantifiable way.
 In this FILM Club he will explain how he has managed to calibrate the fluorescence signal, which dyes turned out best for measurement of the acidic lysosomes, and obviously what interesting results he achieved with all this.

???  Harriet Taylor
(Maggie Dallman lab)
live imaging of zebra fish embryos 
???  Alessandro Pristera
(Kenji Okuse lab) 
high-speed Ca2+ imaging in neurons 
???  Felix Munkonge
(Eric Alton lab) 
labelling of proteins with tetra-Cys markers 
???  Marguerite Wasowicz
(Eric Alton lab) 
clinical-quality standardisation of confocal microscopy acquisition and data analysis 
???  Mark Scott (FILM)  in vivo imaging 
     
     
     

 

Microscope tests

 We are currently organising test installations of widefield and confocal microscopes for the L-Block on Hammersmith Campus. Everybody is welcome to book a time slot (email Martin) to test the systems with their own samples - the more feedback we get the better.

 The systems we are testing are high-sensitivity confocal and widefield microscopes with motorised stage, incubation system and a large range of lasers / illumination.

Schedule:

  • Zeiss confocal LSM-780: Tue-Thu 13-15 September 2011
  • Leica confocal SP5 and widefield: Tue-Thu 11-13 October 2011
  • Zeiss widefield: Tue-Thu 18-20 October 2011
  • Olympus widefield: cancelled by Olympus, tested in Oxford
  • Nikon: cancelled by Nikon

Superresolution microscope (WF3)

 We have now a superresolution microscope in the facility, which is routinely used both for TIRF and PALM single-molecule microscopy with photoswitchable proteins and antibodies, with top quality lens, lasers and camera! For details see Equipment / Widefield 3.

see also Links&Events for external events.

for previous news see FILM News Archive